Construct: shRNA TRCN0000338264

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002833.2-2745s21c1
DNA Barcode:
GAGTGCCAAGCACGTTTATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PTPN9 (5780)
Gene Description:
protein tyrosine phosphatase non-receptor type 9
Transcript:
RefSeq NM_002833.2 (NON-CURRENT)
Match location:
Position 2745 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000338264)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGTGCCAAGCACGTTTATAA
Hairpin Sequence:
5'-CCGG-GAGTGCCAAGCACGTTTATAA-CTCGAG-TTATAAACGTGCTTGGCACTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGTGCCAAGCACGTTTATAACTCGAGTTATAAACGTGCTTGGCACTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGTGCCAAGCACGTTTATAACTCGAGTTATAAACGTGCTTGGCACTC-3'

Other clones with same target sequence:

(none)