Construct: shRNA TRCN0000338276

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000244.3-2202s21c1
DNA Barcode:
CCTCACGCTGGGTCCTAATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MEN1 (4221)
Gene Description:
menin 1
Transcript:
RefSeq NM_000244.3 (CURRENT)
Match location:
Position 2202 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000338276)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCACGCTGGGTCCTAATTA
Hairpin Sequence:
5'-CCGG-CCTCACGCTGGGTCCTAATTA-CTCGAG-TAATTAGGACCCAGCGTGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCACGCTGGGTCCTAATTACTCGAGTAATTAGGACCCAGCGTGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCACGCTGGGTCCTAATTACTCGAGTAATTAGGACCCAGCGTGAGG-3'

Other clones with same target sequence:

(none)