Construct: shRNA TRCN0000338334

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002836.3-3201s21c1
DNA Barcode:
TAGGCTTCTATTACCTATTAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PTPRA (5786)
Gene Description:
protein tyrosine phosphatase receptor type A
Transcript:
RefSeq NM_002836.3 (CURRENT)
Match location:
Position 3201 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000338334)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TAGGCTTCTATTACCTATTAG
Hairpin Sequence:
5'-CCGG-TAGGCTTCTATTACCTATTAG-CTCGAG-CTAATAGGTAATAGAAGCCTA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTAGGCTTCTATTACCTATTAGCTCGAGCTAATAGGTAATAGAAGCCTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATAGGCTTCTATTACCTATTAGCTCGAGCTAATAGGTAATAGAAGCCTA-3'

Other clones with same target sequence:

(none)