Construct: shRNA TRCN0000338372

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015277.4-527s21c1
DNA Barcode:
TCTAATCACAGACTCCTATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NEDD4L (23327)
Gene Description:
NEDD4 like E3 ubiquitin protein ligase
Transcript:
RefSeq NM_015277.4 (NON-CURRENT)
Match location:
Position 527 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000338372)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCTAATCACAGACTCCTATTT
Hairpin Sequence:
5'-CCGG-TCTAATCACAGACTCCTATTT-CTCGAG-AAATAGGAGTCTGTGATTAGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCTAATCACAGACTCCTATTTCTCGAGAAATAGGAGTCTGTGATTAGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCTAATCACAGACTCCTATTTCTCGAGAAATAGGAGTCTGTGATTAGA-3'

Other clones with same target sequence:

(none)