Construct: shRNA TRCN0000338378

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001760.3-634s21c1
DNA Barcode:
GCACATGATTTCCTGGCCTTC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CCND3 (896)
Gene Description:
cyclin D3
Transcript:
RefSeq NM_001760.3 (NON-CURRENT)
Match location:
Position 634 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000338378)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACATGATTTCCTGGCCTTC
Hairpin Sequence:
5'-CCGG-GCACATGATTTCCTGGCCTTC-CTCGAG-GAAGGCCAGGAAATCATGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACATGATTTCCTGGCCTTCCTCGAGGAAGGCCAGGAAATCATGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACATGATTTCCTGGCCTTCCTCGAGGAAGGCCAGGAAATCATGTGC-3'

Other clones with same target sequence:

  1. TRCN0000003828