Construct: shRNA TRCN0000338389

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002835.3-820s21c1
DNA Barcode:
CATAGATTATACGTGGAATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PTPN12 (5782)
Gene Description:
protein tyrosine phosphatase non-receptor type 12
Transcript:
RefSeq NM_002835.3 (NON-CURRENT)
Match location:
Position 820 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000338389)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATAGATTATACGTGGAATTT
Hairpin Sequence:
5'-CCGG-CATAGATTATACGTGGAATTT-CTCGAG-AAATTCCACGTATAATCTATG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATAGATTATACGTGGAATTTCTCGAGAAATTCCACGTATAATCTATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATAGATTATACGTGGAATTTCTCGAGAAATTCCACGTATAATCTATG-3'

Other clones with same target sequence:

(none)