Construct: shRNA TRCN0000338469

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003559.4-1789s21c1
DNA Barcode:
GTTAGGGAGAAGGGTGTATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PIP4K2B (8396)
Gene Description:
phosphatidylinositol-5-phosphate 4-kinase type 2 beta
Transcript:
RefSeq NM_003559.4 (CURRENT)
Match location:
Position 1789 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000338469)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTAGGGAGAAGGGTGTATTT
Hairpin Sequence:
5'-CCGG-GTTAGGGAGAAGGGTGTATTT-CTCGAG-AAATACACCCTTCTCCCTAAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTAGGGAGAAGGGTGTATTTCTCGAGAAATACACCCTTCTCCCTAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTAGGGAGAAGGGTGTATTTCTCGAGAAATACACCCTTCTCCCTAAC-3'

Other clones with same target sequence:

(none)