Construct: shRNA TRCN0000338494

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032408.2-1653s21c1
DNA Barcode:
GCAGATGACTTTGTTGGATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BAZ1B (9031)
Gene Description:
bromodomain adjacent to zinc finger domain 1B
Transcript:
RefSeq NM_032408.2 (NON-CURRENT)
Match location:
Position 1653 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000338494)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGATGACTTTGTTGGATAT
Hairpin Sequence:
5'-CCGG-GCAGATGACTTTGTTGGATAT-CTCGAG-ATATCCAACAAAGTCATCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGATGACTTTGTTGGATATCTCGAGATATCCAACAAAGTCATCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGATGACTTTGTTGGATATCTCGAGATATCCAACAAAGTCATCTGC-3'

Other clones with same target sequence:

  1. TRCN0000013341