Construct: shRNA TRCN0000338520

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015332.3-3265s21c1
DNA Barcode:
CCCTGCTTTAATAAACAGCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NUDCD3 (23386)
Gene Description:
NudC domain containing 3
Transcript:
RefSeq NM_015332.3 (NON-CURRENT)
Match location:
Position 3265 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000338520)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTGCTTTAATAAACAGCAA
Hairpin Sequence:
5'-CCGG-CCCTGCTTTAATAAACAGCAA-CTCGAG-TTGCTGTTTATTAAAGCAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTGCTTTAATAAACAGCAACTCGAGTTGCTGTTTATTAAAGCAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTGCTTTAATAAACAGCAACTCGAGTTGCTGTTTATTAAAGCAGGG-3'

Other clones with same target sequence:

  1. TRCN0000134970