Construct: shRNA TRCN0000338531

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_181472.1-375s21c1
DNA Barcode:
CTGCTGAAAGTGGCGCAAATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CMTM7 (112616)
Gene Description:
CKLF like MARVEL transmembrane domain containing 7
Transcript:
RefSeq NM_181472.1 (NON-CURRENT)
Match location:
Position 375 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000338531)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGCTGAAAGTGGCGCAAATG
Hairpin Sequence:
5'-CCGG-CTGCTGAAAGTGGCGCAAATG-CTCGAG-CATTTGCGCCACTTTCAGCAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGCTGAAAGTGGCGCAAATGCTCGAGCATTTGCGCCACTTTCAGCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGCTGAAAGTGGCGCAAATGCTCGAGCATTTGCGCCACTTTCAGCAG-3'

Other clones with same target sequence:

(none)