Construct: shRNA TRCN0000338568

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003287.2-1080s21c1
DNA Barcode:
AGGTACACAACTGGTCATAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TPD52L1 (7164)
Gene Description:
TPD52 like 1
Transcript:
RefSeq NM_003287.2 (NON-CURRENT)
Match location:
Position 1080 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000338568)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGTACACAACTGGTCATAAT
Hairpin Sequence:
5'-CCGG-AGGTACACAACTGGTCATAAT-CTCGAG-ATTATGACCAGTTGTGTACCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGTACACAACTGGTCATAATCTCGAGATTATGACCAGTTGTGTACCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGTACACAACTGGTCATAATCTCGAGATTATGACCAGTTGTGTACCT-3'

Other clones with same target sequence:

(none)