Construct: shRNA TRCN0000338718

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024713.2-1512s21c1
DNA Barcode:
CTTAAATGTGTGCAGCTATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KATNBL1 (79768)
Gene Description:
katanin regulatory subunit B1 like 1
Transcript:
RefSeq NM_024713.2 (NON-CURRENT)
Match location:
Position 1512 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000338718)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTAAATGTGTGCAGCTATTT
Hairpin Sequence:
5'-CCGG-CTTAAATGTGTGCAGCTATTT-CTCGAG-AAATAGCTGCACACATTTAAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTAAATGTGTGCAGCTATTTCTCGAGAAATAGCTGCACACATTTAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTAAATGTGTGCAGCTATTTCTCGAGAAATAGCTGCACACATTTAAG-3'

Other clones with same target sequence:

(none)