Construct: shRNA TRCN0000338725

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015027.2-2547s21c1
DNA Barcode:
GAAGATGACCACTCACAGGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PDXDC1 (23042)
Gene Description:
pyridoxal dependent decarboxylase domain containing 1
Transcript:
RefSeq NM_015027.2 (NON-CURRENT)
Match location:
Position 2547 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000338725)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGATGACCACTCACAGGTA
Hairpin Sequence:
5'-CCGG-GAAGATGACCACTCACAGGTA-CTCGAG-TACCTGTGAGTGGTCATCTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGATGACCACTCACAGGTACTCGAGTACCTGTGAGTGGTCATCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGATGACCACTCACAGGTACTCGAGTACCTGTGAGTGGTCATCTTC-3'

Other clones with same target sequence:

  1. TRCN0000163453