Construct: shRNA TRCN0000338742

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015263.2-8785s21c1
DNA Barcode:
GCCACAAGTGACTTTGCATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DMXL2 (23312)
Gene Description:
Dmx like 2
Transcript:
RefSeq NM_015263.2 (NON-CURRENT)
Match location:
Position 8785 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000338742)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCACAAGTGACTTTGCATTT
Hairpin Sequence:
5'-CCGG-GCCACAAGTGACTTTGCATTT-CTCGAG-AAATGCAAAGTCACTTGTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCACAAGTGACTTTGCATTTCTCGAGAAATGCAAAGTCACTTGTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCACAAGTGACTTTGCATTTCTCGAGAAATGCAAAGTCACTTGTGGC-3'

Other clones with same target sequence:

  1. TRCN0000142512