Construct: shRNA TRCN0000338830

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015024.4-545s21c1
DNA Barcode:
GCATCATTGGTGTCACAATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
XPO7 (23039)
Gene Description:
exportin 7
Transcript:
RefSeq NM_015024.4 (NON-CURRENT)
Match location:
Position 545 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000338830)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATCATTGGTGTCACAATTT
Hairpin Sequence:
5'-CCGG-GCATCATTGGTGTCACAATTT-CTCGAG-AAATTGTGACACCAATGATGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATCATTGGTGTCACAATTTCTCGAGAAATTGTGACACCAATGATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATCATTGGTGTCACAATTTCTCGAGAAATTGTGACACCAATGATGC-3'

Other clones with same target sequence:

  1. TRCN0000159944