Construct: shRNA TRCN0000338858

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021806.1-874s21c1
DNA Barcode:
GCACGTGAAGAACAGTAAGCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FAM3A (60343)
Gene Description:
FAM3 metabolism regulating signaling molecule A
Transcript:
RefSeq NM_021806.1 (NON-CURRENT)
Match location:
Position 874 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000338858)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACGTGAAGAACAGTAAGCA
Hairpin Sequence:
5'-CCGG-GCACGTGAAGAACAGTAAGCA-CTCGAG-TGCTTACTGTTCTTCACGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACGTGAAGAACAGTAAGCACTCGAGTGCTTACTGTTCTTCACGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACGTGAAGAACAGTAAGCACTCGAGTGCTTACTGTTCTTCACGTGC-3'

Other clones with same target sequence:

  1. TRCN0000157661