Construct: shRNA TRCN0000339084

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009535.2-1934s21c1
DNA Barcode:
GCTGCTCTGTATGGTCGATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Yes1 (22612)
Gene Description:
YES proto-oncogene 1, Src family tyrosine kinase
Transcript:
RefSeq NM_009535.2 (NON-CURRENT)
Match location:
Position 1934 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000339084)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGCTCTGTATGGTCGATTT
Hairpin Sequence:
5'-CCGG-GCTGCTCTGTATGGTCGATTT-CTCGAG-AAATCGACCATACAGAGCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGCTCTGTATGGTCGATTTCTCGAGAAATCGACCATACAGAGCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGCTCTGTATGGTCGATTTCTCGAGAAATCGACCATACAGAGCAGC-3'

Other clones with same target sequence:

  1. TRCN0000023616