Construct: shRNA TRCN0000339107

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145514.4-820s21c1
DNA Barcode:
CAAAGGGATCGGTGCCTATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Wdr26 (226757)
Gene Description:
WD repeat domain 26
Transcript:
RefSeq NM_145514.4 (NON-CURRENT)
Match location:
Position 820 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000339107)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAAGGGATCGGTGCCTATAT
Hairpin Sequence:
5'-CCGG-CAAAGGGATCGGTGCCTATAT-CTCGAG-ATATAGGCACCGATCCCTTTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAAGGGATCGGTGCCTATATCTCGAGATATAGGCACCGATCCCTTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAAGGGATCGGTGCCTATATCTCGAGATATAGGCACCGATCCCTTTG-3'

Other clones with same target sequence:

  1. TRCN0000139717