Construct: shRNA TRCN0000339109

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_199195.1-840s21c1
DNA Barcode:
GTCATCGATCTGCGGACAATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Bckdhb (12040)
Gene Description:
branched chain ketoacid dehydrogenase E1, beta polypeptide
Transcript:
RefSeq NM_199195.1 (CURRENT)
Match location:
Position 840 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000339109)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCATCGATCTGCGGACAATT
Hairpin Sequence:
5'-CCGG-GTCATCGATCTGCGGACAATT-CTCGAG-AATTGTCCGCAGATCGATGAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCATCGATCTGCGGACAATTCTCGAGAATTGTCCGCAGATCGATGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCATCGATCTGCGGACAATTCTCGAGAATTGTCCGCAGATCGATGAC-3'

Other clones with same target sequence:

(none)