Construct: shRNA TRCN0000339168

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145514.4-1507s21c1
DNA Barcode:
GCTTTGTTAAATGTAGCAACT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Wdr26 (226757)
Gene Description:
WD repeat domain 26
Transcript:
RefSeq NM_145514.4 (NON-CURRENT)
Match location:
Position 1507 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000339168)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTTGTTAAATGTAGCAACT
Hairpin Sequence:
5'-CCGG-GCTTTGTTAAATGTAGCAACT-CTCGAG-AGTTGCTACATTTAACAAAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTTGTTAAATGTAGCAACTCTCGAGAGTTGCTACATTTAACAAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTTGTTAAATGTAGCAACTCTCGAGAGTTGCTACATTTAACAAAGC-3'

Other clones with same target sequence:

  1. TRCN0000177411