Construct: shRNA TRCN0000339176

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001146342.1-134s21c1
DNA Barcode:
CTGCCAGCAGTCCTCATAATC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rnls (67795)
Gene Description:
renalase, FAD-dependent amine oxidase
Transcript:
RefSeq NM_001146342.1 (NON-CURRENT)
Match location:
Position 134 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000339176)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGCCAGCAGTCCTCATAATC
Hairpin Sequence:
5'-CCGG-CTGCCAGCAGTCCTCATAATC-CTCGAG-GATTATGAGGACTGCTGGCAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGCCAGCAGTCCTCATAATCCTCGAGGATTATGAGGACTGCTGGCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGCCAGCAGTCCTCATAATCCTCGAGGATTATGAGGACTGCTGGCAG-3'

Other clones with same target sequence:

(none)