Construct: shRNA TRCN0000339207

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028106.2-605s21c1
DNA Barcode:
TCTGAAGCAGGAGGCACAATC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zbed3 (72114)
Gene Description:
zinc finger, BED type containing 3
Transcript:
RefSeq NM_028106.2 (NON-CURRENT)
Match location:
Position 605 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000339207)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCTGAAGCAGGAGGCACAATC
Hairpin Sequence:
5'-CCGG-TCTGAAGCAGGAGGCACAATC-CTCGAG-GATTGTGCCTCCTGCTTCAGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCTGAAGCAGGAGGCACAATCCTCGAGGATTGTGCCTCCTGCTTCAGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCTGAAGCAGGAGGCACAATCCTCGAGGATTGTGCCTCCTGCTTCAGA-3'

Other clones with same target sequence:

(none)