Construct: shRNA TRCN0000339215

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144844.2-1664s21c1
DNA Barcode:
ATTCAAGAGTACCAGTTATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pcca (110821)
Gene Description:
propionyl-Coenzyme A carboxylase, alpha polypeptide
Transcript:
RefSeq NM_144844.2 (CURRENT)
Match location:
Position 1664 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000339215)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATTCAAGAGTACCAGTTATTA
Hairpin Sequence:
5'-CCGG-ATTCAAGAGTACCAGTTATTA-CTCGAG-TAATAACTGGTACTCTTGAAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATTCAAGAGTACCAGTTATTACTCGAGTAATAACTGGTACTCTTGAATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATTCAAGAGTACCAGTTATTACTCGAGTAATAACTGGTACTCTTGAAT-3'

Other clones with same target sequence:

(none)