Construct: shRNA TRCN0000339297

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019678.2-540s21c1
DNA Barcode:
ACTGGTAGAACTTCGAAATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tfg (21787)
Gene Description:
Trk-fused gene
Transcript:
RefSeq NM_019678.2 (NON-CURRENT)
Match location:
Position 540 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000339297)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACTGGTAGAACTTCGAAATAA
Hairpin Sequence:
5'-CCGG-ACTGGTAGAACTTCGAAATAA-CTCGAG-TTATTTCGAAGTTCTACCAGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACTGGTAGAACTTCGAAATAACTCGAGTTATTTCGAAGTTCTACCAGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACTGGTAGAACTTCGAAATAACTCGAGTTATTTCGAAGTTCTACCAGT-3'

Other clones with same target sequence:

(none)