Construct: shRNA TRCN0000339420

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172645.2-2821s21c1
DNA Barcode:
TAGTATAGCTGATACACTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Suco (226551)
Gene Description:
SUN domain containing ossification factor
Transcript:
RefSeq NM_172645.2 (CURRENT)
Match location:
Position 2821 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000339420)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TAGTATAGCTGATACACTAAA
Hairpin Sequence:
5'-CCGG-TAGTATAGCTGATACACTAAA-CTCGAG-TTTAGTGTATCAGCTATACTA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTAGTATAGCTGATACACTAAACTCGAGTTTAGTGTATCAGCTATACTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATAGTATAGCTGATACACTAAACTCGAGTTTAGTGTATCAGCTATACTA-3'

Other clones with same target sequence:

(none)