Construct: shRNA TRCN0000339623

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144911.2-1651s21c1
DNA Barcode:
AGATTACAATGGGAGATATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rpap2 (231571)
Gene Description:
RNA polymerase II associated protein 2
Transcript:
RefSeq NM_144911.2 (NON-CURRENT)
Match location:
Position 1651 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000339623)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGATTACAATGGGAGATATTT
Hairpin Sequence:
5'-CCGG-AGATTACAATGGGAGATATTT-CTCGAG-AAATATCTCCCATTGTAATCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGATTACAATGGGAGATATTTCTCGAGAAATATCTCCCATTGTAATCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGATTACAATGGGAGATATTTCTCGAGAAATATCTCCCATTGTAATCT-3'

Other clones with same target sequence:

(none)