Construct: shRNA TRCN0000339701

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172407.3-445s21c1
DNA Barcode:
GTATGGCTGAAGGCATCAAAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cdkn2aip (70925)
Gene Description:
CDKN2A interacting protein
Transcript:
RefSeq NM_172407.3 (CURRENT)
Match location:
Position 445 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000339701)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTATGGCTGAAGGCATCAAAG
Hairpin Sequence:
5'-CCGG-GTATGGCTGAAGGCATCAAAG-CTCGAG-CTTTGATGCCTTCAGCCATAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTATGGCTGAAGGCATCAAAGCTCGAGCTTTGATGCCTTCAGCCATACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTATGGCTGAAGGCATCAAAGCTCGAGCTTTGATGCCTTCAGCCATAC-3'

Other clones with same target sequence:

(none)