Construct: shRNA TRCN0000339798

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009836.1-1804s21c1
DNA Barcode:
AGGAACACTGTGGACATCTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cct3 (12462)
Gene Description:
chaperonin containing Tcp1, subunit 3 (gamma)
Transcript:
RefSeq NM_009836.1 (CURRENT)
Match location:
Position 1804 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000339798)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGAACACTGTGGACATCTTT
Hairpin Sequence:
5'-CCGG-AGGAACACTGTGGACATCTTT-CTCGAG-AAAGATGTCCACAGTGTTCCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGAACACTGTGGACATCTTTCTCGAGAAAGATGTCCACAGTGTTCCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGAACACTGTGGACATCTTTCTCGAGAAAGATGTCCACAGTGTTCCT-3'

Other clones with same target sequence:

  1. TRCN0000120447