Construct: shRNA TRCN0000339830

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021493.2-3073s21c1
DNA Barcode:
AGTACCTCCTGCCCAACATTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Arhgap23 (58996)
Gene Description:
Rho GTPase activating protein 23
Transcript:
RefSeq NM_021493.2 (CURRENT)
Match location:
Position 3073 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000339830)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGTACCTCCTGCCCAACATTG
Hairpin Sequence:
5'-CCGG-AGTACCTCCTGCCCAACATTG-CTCGAG-CAATGTTGGGCAGGAGGTACT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGTACCTCCTGCCCAACATTGCTCGAGCAATGTTGGGCAGGAGGTACTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGTACCTCCTGCCCAACATTGCTCGAGCAATGTTGGGCAGGAGGTACT-3'

Other clones with same target sequence:

(none)