Construct: shRNA TRCN0000339834

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145471.2-852s21c1
DNA Barcode:
CCGAGTAGACCTTCGGGTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Lrrc14 (223664)
Gene Description:
leucine rich repeat containing 14
Transcript:
RefSeq NM_145471.2 (CURRENT)
Match location:
Position 852 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000339834)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGAGTAGACCTTCGGGTAAA
Hairpin Sequence:
5'-CCGG-CCGAGTAGACCTTCGGGTAAA-CTCGAG-TTTACCCGAAGGTCTACTCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGAGTAGACCTTCGGGTAAACTCGAGTTTACCCGAAGGTCTACTCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGAGTAGACCTTCGGGTAAACTCGAGTTTACCCGAAGGTCTACTCGG-3'

Other clones with same target sequence:

(none)