Construct: shRNA TRCN0000339895

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026592.3-885s21c1
DNA Barcode:
GTTCACACTCAAGCGACATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
B230118H07Rik (68170)
Gene Description:
RIKEN cDNA B230118H07 gene
Transcript:
RefSeq NM_026592.3 (CURRENT)
Match location:
Position 885 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000339895)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTCACACTCAAGCGACATTA
Hairpin Sequence:
5'-CCGG-GTTCACACTCAAGCGACATTA-CTCGAG-TAATGTCGCTTGAGTGTGAAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTCACACTCAAGCGACATTACTCGAGTAATGTCGCTTGAGTGTGAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTCACACTCAAGCGACATTACTCGAGTAATGTCGCTTGAGTGTGAAC-3'

Other clones with same target sequence:

(none)