Construct: shRNA TRCN0000340039

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_207239.1-6553s21c1
DNA Barcode:
CAGCAGGGTGGATGTTGTTTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gtf3c1 (233863)
Gene Description:
general transcription factor III C 1
Transcript:
RefSeq NM_207239.1 (CURRENT)
Match location:
Position 6553 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000340039)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGCAGGGTGGATGTTGTTTG
Hairpin Sequence:
5'-CCGG-CAGCAGGGTGGATGTTGTTTG-CTCGAG-CAAACAACATCCACCCTGCTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGCAGGGTGGATGTTGTTTGCTCGAGCAAACAACATCCACCCTGCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGCAGGGTGGATGTTGTTTGCTCGAGCAAACAACATCCACCCTGCTG-3'

Other clones with same target sequence:

(none)