Construct: shRNA TRCN0000340185

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008515.4-534s21c1
DNA Barcode:
ACGGTTATGACGGAGACTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Lrrfip1 (16978)
Gene Description:
leucine rich repeat (in FLII) interacting protein 1
Transcript:
RefSeq NM_008515.4 (CURRENT)
Match location:
Position 534 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000340185)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACGGTTATGACGGAGACTATT
Hairpin Sequence:
5'-CCGG-ACGGTTATGACGGAGACTATT-CTCGAG-AATAGTCTCCGTCATAACCGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACGGTTATGACGGAGACTATTCTCGAGAATAGTCTCCGTCATAACCGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACGGTTATGACGGAGACTATTCTCGAGAATAGTCTCCGTCATAACCGT-3'

Other clones with same target sequence:

(none)