Construct: shRNA TRCN0000340239

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001025307.1-1480s21c1
DNA Barcode:
GATCATGATCTGCTGTATTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Stx3 (20908)
Gene Description:
syntaxin 3
Transcript:
RefSeq NM_001025307.1 (CURRENT)
Match location:
Position 1480 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000340239)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATCATGATCTGCTGTATTAT
Hairpin Sequence:
5'-CCGG-GATCATGATCTGCTGTATTAT-CTCGAG-ATAATACAGCAGATCATGATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATCATGATCTGCTGTATTATCTCGAGATAATACAGCAGATCATGATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATCATGATCTGCTGTATTATCTCGAGATAATACAGCAGATCATGATC-3'

Other clones with same target sequence:

(none)