Construct: shRNA TRCN0000340363

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030179.3-2139s21c1
DNA Barcode:
TGTGGGTGACAAGCGCTATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Clip4 (78785)
Gene Description:
CAP-GLY domain containing linker protein family, member 4
Transcript:
RefSeq NM_030179.3 (CURRENT)
Match location:
Position 2139 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000340363)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGTGGGTGACAAGCGCTATTT
Hairpin Sequence:
5'-CCGG-TGTGGGTGACAAGCGCTATTT-CTCGAG-AAATAGCGCTTGTCACCCACA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGTGGGTGACAAGCGCTATTTCTCGAGAAATAGCGCTTGTCACCCACATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGTGGGTGACAAGCGCTATTTCTCGAGAAATAGCGCTTGTCACCCACA-3'

Other clones with same target sequence:

(none)