Construct: shRNA TRCN0000340370

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022019.5-1292s21c1
DNA Barcode:
AGAACCTGCGGCAGTACTTTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dusp10 (63953)
Gene Description:
dual specificity phosphatase 10
Transcript:
RefSeq NM_022019.5 (CURRENT)
Match location:
Position 1292 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000340370)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGAACCTGCGGCAGTACTTTG
Hairpin Sequence:
5'-CCGG-AGAACCTGCGGCAGTACTTTG-CTCGAG-CAAAGTACTGCCGCAGGTTCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGAACCTGCGGCAGTACTTTGCTCGAGCAAAGTACTGCCGCAGGTTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGAACCTGCGGCAGTACTTTGCTCGAGCAAAGTACTGCCGCAGGTTCT-3'

Other clones with same target sequence:

  1. TRCN0000314698