Construct: shRNA TRCN0000340564

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145150.2-1835s21c1
DNA Barcode:
GTCTGTTGCCAGCACCTATTC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Prc1 (233406)
Gene Description:
protein regulator of cytokinesis 1
Transcript:
RefSeq NM_145150.2 (NON-CURRENT)
Match location:
Position 1835 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000340564)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCTGTTGCCAGCACCTATTC
Hairpin Sequence:
5'-CCGG-GTCTGTTGCCAGCACCTATTC-CTCGAG-GAATAGGTGCTGGCAACAGAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCTGTTGCCAGCACCTATTCCTCGAGGAATAGGTGCTGGCAACAGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCTGTTGCCAGCACCTATTCCTCGAGGAATAGGTGCTGGCAACAGAC-3'

Other clones with same target sequence:

(none)