Construct: shRNA TRCN0000340658

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177448.4-1505s21c1
DNA Barcode:
GGGTCCTCTGAGCTATGATAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mogat2 (233549)
Gene Description:
monoacylglycerol O-acyltransferase 2
Transcript:
RefSeq NM_177448.4 (CURRENT)
Match location:
Position 1505 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000340658)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGGTCCTCTGAGCTATGATAC
Hairpin Sequence:
5'-CCGG-GGGTCCTCTGAGCTATGATAC-CTCGAG-GTATCATAGCTCAGAGGACCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGGTCCTCTGAGCTATGATACCTCGAGGTATCATAGCTCAGAGGACCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGGTCCTCTGAGCTATGATACCTCGAGGTATCATAGCTCAGAGGACCC-3'

Other clones with same target sequence:

(none)