Construct: shRNA TRCN0000340676

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009201.2-1226s21c1
DNA Barcode:
GTATCCAGCGGGAGATCAATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc1a5 (20514)
Gene Description:
solute carrier family 1 (neutral amino acid transporter), member 5
Transcript:
RefSeq NM_009201.2 (CURRENT)
Match location:
Position 1226 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000340676)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTATCCAGCGGGAGATCAATT
Hairpin Sequence:
5'-CCGG-GTATCCAGCGGGAGATCAATT-CTCGAG-AATTGATCTCCCGCTGGATAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTATCCAGCGGGAGATCAATTCTCGAGAATTGATCTCCCGCTGGATACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTATCCAGCGGGAGATCAATTCTCGAGAATTGATCTCCCGCTGGATAC-3'

Other clones with same target sequence:

(none)