Construct: shRNA TRCN0000340687

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178218.3-208s21c1
DNA Barcode:
TGGCGGCTGTGCTAGAATATC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hist3h2a (319162)
Gene Description:
histone cluster 3, H2a
Transcript:
RefSeq NM_178218.3 (NON-CURRENT)
Match location:
Position 208 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000340687)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGGCGGCTGTGCTAGAATATC
Hairpin Sequence:
5'-CCGG-TGGCGGCTGTGCTAGAATATC-CTCGAG-GATATTCTAGCACAGCCGCCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGGCGGCTGTGCTAGAATATCCTCGAGGATATTCTAGCACAGCCGCCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGGCGGCTGTGCTAGAATATCCTCGAGGATATTCTAGCACAGCCGCCA-3'

Other clones with same target sequence:

(none)