Construct: shRNA TRCN0000340699

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_138755.2-2226s21c1
DNA Barcode:
CCCGAGTTTGCCAATTGATTC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Phf21a (192285)
Gene Description:
PHD finger protein 21A
Transcript:
RefSeq NM_138755.2 (CURRENT)
Match location:
Position 2226 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000340699)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCGAGTTTGCCAATTGATTC
Hairpin Sequence:
5'-CCGG-CCCGAGTTTGCCAATTGATTC-CTCGAG-GAATCAATTGGCAAACTCGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCGAGTTTGCCAATTGATTCCTCGAGGAATCAATTGGCAAACTCGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCGAGTTTGCCAATTGATTCCTCGAGGAATCAATTGGCAAACTCGGG-3'

Other clones with same target sequence:

(none)