Construct: shRNA TRCN0000340712

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030225.3-132s21c1
DNA Barcode:
ACAACAGGAAGATGGTCATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dlst (78920)
Gene Description:
dihydrolipoamide S-succinyltransferase (E2 component of 2-oxo-glutarate complex)
Transcript:
RefSeq NM_030225.3 (NON-CURRENT)
Match location:
Position 132 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000340712)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACAACAGGAAGATGGTCATTA
Hairpin Sequence:
5'-CCGG-ACAACAGGAAGATGGTCATTA-CTCGAG-TAATGACCATCTTCCTGTTGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACAACAGGAAGATGGTCATTACTCGAGTAATGACCATCTTCCTGTTGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACAACAGGAAGATGGTCATTACTCGAGTAATGACCATCTTCCTGTTGT-3'

Other clones with same target sequence:

(none)