Construct: shRNA TRCN0000340762

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019460.1-174s21c1
DNA Barcode:
GTTCCCTACGCATCGTTTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sfmbt1 (54650)
Gene Description:
Scm-like with four mbt domains 1
Transcript:
RefSeq NM_019460.1 (NON-CURRENT)
Match location:
Position 174 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000340762)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTCCCTACGCATCGTTTAAA
Hairpin Sequence:
5'-CCGG-GTTCCCTACGCATCGTTTAAA-CTCGAG-TTTAAACGATGCGTAGGGAAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTCCCTACGCATCGTTTAAACTCGAGTTTAAACGATGCGTAGGGAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTCCCTACGCATCGTTTAAACTCGAGTTTAAACGATGCGTAGGGAAC-3'

Other clones with same target sequence:

(none)