Construct: shRNA TRCN0000340819

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145221.2-220s21c1
DNA Barcode:
TCAAGCTATGCATCGGATTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Appl1 (72993)
Gene Description:
adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1
Transcript:
RefSeq NM_145221.2 (CURRENT)
Match location:
Position 220 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000340819)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCAAGCTATGCATCGGATTTA
Hairpin Sequence:
5'-CCGG-TCAAGCTATGCATCGGATTTA-CTCGAG-TAAATCCGATGCATAGCTTGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCAAGCTATGCATCGGATTTACTCGAGTAAATCCGATGCATAGCTTGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCAAGCTATGCATCGGATTTACTCGAGTAAATCCGATGCATAGCTTGA-3'

Other clones with same target sequence:

(none)