Construct: shRNA TRCN0000340934

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010837.3-2119s21c1
DNA Barcode:
AGACCTCAACGAGCCATAAAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Map6 (17760)
Gene Description:
microtubule-associated protein 6
Transcript:
RefSeq NM_010837.3 (CURRENT)
Match location:
Position 2119 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000340934)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGACCTCAACGAGCCATAAAC
Hairpin Sequence:
5'-CCGG-AGACCTCAACGAGCCATAAAC-CTCGAG-GTTTATGGCTCGTTGAGGTCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGACCTCAACGAGCCATAAACCTCGAGGTTTATGGCTCGTTGAGGTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGACCTCAACGAGCCATAAACCTCGAGGTTTATGGCTCGTTGAGGTCT-3'

Other clones with same target sequence:

(none)