Construct: shRNA TRCN0000341108

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025638.2-456s21c1
DNA Barcode:
TAAACGTCTCTGATCTCAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gdpd1 (66569)
Gene Description:
glycerophosphodiester phosphodiesterase domain containing 1
Transcript:
RefSeq NM_025638.2 (CURRENT)
Match location:
Position 456 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000341108)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TAAACGTCTCTGATCTCAAAT
Hairpin Sequence:
5'-CCGG-TAAACGTCTCTGATCTCAAAT-CTCGAG-ATTTGAGATCAGAGACGTTTA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTAAACGTCTCTGATCTCAAATCTCGAGATTTGAGATCAGAGACGTTTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATAAACGTCTCTGATCTCAAATCTCGAGATTTGAGATCAGAGACGTTTA-3'

Other clones with same target sequence:

(none)