Construct: shRNA TRCN0000341168

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027217.1-969s21c1
DNA Barcode:
GAGGACTTCTGCATCCTTATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
1810046K07Rik (69809)
Gene Description:
RIKEN cDNA 1810046K07 gene
Transcript:
RefSeq NM_027217.1 (CURRENT)
Match location:
Position 969 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000341168)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGGACTTCTGCATCCTTATA
Hairpin Sequence:
5'-CCGG-GAGGACTTCTGCATCCTTATA-CTCGAG-TATAAGGATGCAGAAGTCCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGGACTTCTGCATCCTTATACTCGAGTATAAGGATGCAGAAGTCCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGGACTTCTGCATCCTTATACTCGAGTATAAGGATGCAGAAGTCCTC-3'

Other clones with same target sequence:

(none)