Construct: shRNA TRCN0000341224

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001162906.1-3656s21c1
DNA Barcode:
GGGTATGGACTTACCATTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
2410089E03Rik (73692)
Gene Description:
RIKEN cDNA 2410089E03 gene
Transcript:
RefSeq NM_001162906.1 (CURRENT)
Match location:
Position 3656 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000341224)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGGTATGGACTTACCATTAAA
Hairpin Sequence:
5'-CCGG-GGGTATGGACTTACCATTAAA-CTCGAG-TTTAATGGTAAGTCCATACCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGGTATGGACTTACCATTAAACTCGAGTTTAATGGTAAGTCCATACCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGGTATGGACTTACCATTAAACTCGAGTTTAATGGTAAGTCCATACCC-3'

Other clones with same target sequence:

(none)