Construct: shRNA TRCN0000341296

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027594.1-1263s21c1
DNA Barcode:
CTAAGTTCTGGAGTGATATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ttll7 (70892)
Gene Description:
tubulin tyrosine ligase-like family, member 7
Transcript:
RefSeq NM_027594.1 (NON-CURRENT)
Match location:
Position 1263 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000341296)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTAAGTTCTGGAGTGATATTT
Hairpin Sequence:
5'-CCGG-CTAAGTTCTGGAGTGATATTT-CTCGAG-AAATATCACTCCAGAACTTAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTAAGTTCTGGAGTGATATTTCTCGAGAAATATCACTCCAGAACTTAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTAAGTTCTGGAGTGATATTTCTCGAGAAATATCACTCCAGAACTTAG-3'

Other clones with same target sequence:

(none)