Construct: shRNA TRCN0000341307

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175510.3-10280s21c1
DNA Barcode:
TAATATTACTGCCCTCATAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Unc80 (329178)
Gene Description:
unc-80, NALCN activator
Transcript:
RefSeq NM_175510.3 (CURRENT)
Match location:
Position 10280 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000341307)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TAATATTACTGCCCTCATAAT
Hairpin Sequence:
5'-CCGG-TAATATTACTGCCCTCATAAT-CTCGAG-ATTATGAGGGCAGTAATATTA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTAATATTACTGCCCTCATAATCTCGAGATTATGAGGGCAGTAATATTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATAATATTACTGCCCTCATAATCTCGAGATTATGAGGGCAGTAATATTA-3'

Other clones with same target sequence:

(none)